Milenia® GenLine Tests - Test procedure

  • 01/20

    01/20

    Milenia GenLine Tests are based on a Universal Module (left) and specific PCR Modules (right)

  • 02/20

    02/20

    Testing in liquid culture medium: .....

  • 03/20

    03/20

    ..... Samples from liquid culture medium can be directly introduced in the test.

  • 04/20

    04/20

    Direct analysis of single colonies on solid media: A colony is taken .....

  • 05/20

    05/20

    ..... and suspended in liquid (e.g. PCR-Water).

  • 06/20

    06/20

    2 µL of the prepared sample is pipetted to PCR-tubes.

  • 07/20

    07/20

    2 µL of the prepared sample is pipetted to PCR-tubes.

  • 08/20

    08/20

    Brief centrifugation of PCR-tubes.

  • 09/20

    09/20

    PCR-tubes are transfered to the thermocycler and the program is started.

  • 10/20

    10/20

    PCR-tubes are taken out of the thermocycler.

  • 11/20

    11/20

    Test strips are taken out of the container and .....

  • 12/20

    12/20

    ...... labeled.

  • 13/20

    13/20

    2 µl liquid of the PCR-tube are transfered to the upper part of the sample application area of the test strip.

  • 14/20

    14/20

    80 µl running buffer .....

  • 15/20

    15/20

    ..... are transfered to a microtiter plate.

  • 16/20

    16/20

    Test strips are placed into the wells of the microtiter plate.

  • 17/20

    17/20

    Test strips are incubated for 5 minutes.

  • 18/20

    18/20

    Test strips are fixed to the evaluation sheet and documented.

  • 19/20

    19/20

    Milenia GenLine Tests for the detection of beer spoiling organisms.